Lee, Ha Neul, et al. Chemico-Biological Interactions 219 (2014): 151-158.
Cancer is the leading cause of gynecological malignant tumor mortality. Ethyl caffeate (EC) and its derivatives are natural phenolic compounds isolated from various plants, which have been reported to possess anti-inflammatory and anti-tumor activities.
Ethyl Caffeate Inhibits Cell Proliferation by Cell Cycle Arrest
First, the effect of ethyl caffeate (EC) on the proliferation of SKOV-3 cells was examined. EC treatment inhibited cell proliferation in a dose-dependent manner without altering cell viability, indicating that the EC-mediated inhibition of cell proliferation was not mediated by cytotoxicity. Furthermore, preliminary experiments showed that EC did not induce cell apoptosis. Based on these findings, the regulatory effect of EC on the cell cycle was investigated by DNA content analysis. Compared to the untreated control, 24 hours of mitogenic stimulation increased the percentage of cells in S phase (5.8% vs. 20.1%) and G2/M phase (18.8% vs. 26.9%), and decreased the percentage of cells in G1 phase (70.5% vs. 40.8%). However, similar to the untreated control, EC treatment significantly prevented the increase in S phase (20.1% vs. 11.3%) and G2/M phase (26.9% vs. 15.5%) and the decrease in G1 phase (40.8% vs. 64.6%) associated with mitogenic stimulation. These findings indicate that EC inhibits the G1 to S phase transition of the cell cycle, leading to G1 phase arrest, which is closely related to the previous observations on cell proliferation. It has been reported that cell cycle progression requires the activation of cyclin-dependent kinases (Cdks) by forming complexes with cyclins, and subsequent phosphorylation of retinoblastoma protein (pRb). Therefore, the changes in cell cycle-related proteins were further analyzed. EC treatment dose-dependently suppressed the expression of Cdks and cyclins, leading to hypophosphorylation of pRb to the level observed in the untreated control. Taken together, these findings indicate that EC downregulates the expression of cell cycle-related proteins, thereby inhibiting cell cycle progression and proliferation.
What is the CAS number for Ethyl Caffeate?
The CAS number for Ethyl Caffeate is 102-37-4.
What are some synonyms for Ethyl Caffeate?
Some synonyms for Ethyl Caffeate are 2-Propenoic acid, 3-(3,4-dihydroxyphenyl)-, ethyl ester and Caffeic acid ethyl ester.
What is the molecular weight of Ethyl Caffeate?
The molecular weight of Ethyl Caffeate is 208.21.
What is the purity of Ethyl Caffeate?
The purity of Ethyl Caffeate is 98%.
What is the density of Ethyl Caffeate?
The density of Ethyl Caffeate is 1.271g/cm³.
What percentage of Ethyl Caffeate is considered as actives?
95% of Ethyl Caffeate is considered as actives.
In what physical state is Ethyl Caffeate?
Ethyl Caffeate is in a solid physical state.
What is a typical application of Ethyl Caffeate?
A typical application of Ethyl Caffeate is its use as an antioxidant.
Can Ethyl Caffeate be used as a liquid?
No, Ethyl Caffeate is in a solid physical state, not a liquid.
Is Ethyl Caffeate known for its purity or density?
Ethyl Caffeate is known for its purity, which is 98%.
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